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Part:BBa_K649401:Design

Designed by: Maiko Hayashi   Group: iGEM11_Tokyo_Tech   (2011-09-19)
Revision as of 07:48, 17 February 2012 by Ratha (Talk | contribs) (→‎Design Notes)

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Arg box


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The BioBrick-Prefix of this part is for "ATG-bigging type" while this's started from "AAG."

However, you can use EcoRI/XbaI to construct and this will work correctly (binding site is from 17th bp).

Source

obtained from MG1655(E.coli) by PCR.

References

Stefan Schuster, "A general definition of metabolic pathways useful for systematic organization and analysis of complex metabolic networks" Nature Biotechnology , vol. 18, p326-32 (2000)