Regulatory

Part:BBa_J153001:Design

Designed by: Daniel Camsund / Hsinho Huang   Group: Lindblad Lab   (2012-02-03)
Revision as of 11:58, 3 February 2012 by Daniel Camsund (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Ptrc1O


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Ptrc1O, or the trc promoter, was extracted from the pTrc99A plasmid using PCR (Huang etal 2010, Brosius etal 1985).

References

Brosius, J., Erfle, M. and Storella, J. (1985) Spacing of the -10 and -35 Regions in the Tac Promoter - Effect on Its Invivo Activity. Journal of Biological Chemistry, 260, 3539-3541.

Huang, H.H., Camsund, D., Lindblad, P. and Heidorn, T. (2010) Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology. Nucleic Acids Research, 38, 2577-2593.