Translational_Unit

Part:BBa_K206009:Experience

Designed by: Amelia Hardjasa   Group: iGEM09_British_Columbia   (2009-10-18)
Revision as of 20:32, 2 February 2012 by Alex273 (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K206009

User Reviews

UNIQb6d8b7189189d1d3-partinfo-00000000-QINU UNIQb6d8b7189189d1d3-partinfo-00000001-QINU

Alex273

The plasmid seems to be missing the X and S restriction sites. Digestion by these enzymes fails GelK206009.jpg

Agarose electrophoresis of part K206009 as ordered from the registry in spring 2011 digested with several iGEM enzymes. nd stands for "non digested"

As shown above, the digestion by P linearizes the plasmid as expected but X and S have no effect compared to the non digested plasmid. Moreover, double digestion by X+P only linearizes the plasmid.