Plasmid_Backbone

Part:BBa_J176124

Designed by: Karmella Haynes, Andrew Younger   Group: Haynes Lab   (2011-10-22)
Revision as of 02:57, 8 January 2012 by Kahaynes (Talk | contribs) (→‎Usage and Biology)

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pcDNA3.1+ zeo

pcDNA3.1+ zeo/ ΔpCMV, based on pcDNA3.1+; no promoter; ampicillin and zeocin resistance
Aliases: AY2, MV8



Usage and Biology

  • Select with ampicillin in E. coli
  • Select with zeocin in mammalian cells

Mammalian DNA transfection vector. Great for temporary expression of your gene of interest, but transfection isn't 100% (for us, around 10%). The zeocin marker enables zeocin selection of mammalian cells that carry the plasmid. Long term selection leads to random insertion: a population of cells that have the plasmid (or multiple copies) inserted into a random spot in one or more chromosomes.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
    Illegal NotI site found at 292
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal BglII site found at 12
    Illegal BamHI site found at 1438
    Illegal XhoI site found at 299
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
    Illegal NgoMIV site found at 737
    Illegal NgoMIV site found at 1887
    Illegal NgoMIV site found at 1948
    Illegal NgoMIV site found at 2213
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 3738
    Illegal SapI site found at 2658


[edit]
Categories
Parameters
n/apcDNA3.1+ zeo