Coding

Part:BBa_M36251:Design

Designed by: Evan Masutani, Erica Lieberman   Group: Stanford BIOE44 - S11   (2011-12-09)
Revision as of 18:24, 9 December 2011 by Registry (Talk | contribs)

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AlkS Transcription Factor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 262
    Illegal EcoRI site found at 475
    Illegal PstI site found at 991
    Illegal PstI site found at 1615
    Illegal PstI site found at 2023
    Illegal PstI site found at 2299
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 262
    Illegal EcoRI site found at 475
    Illegal PstI site found at 991
    Illegal PstI site found at 1615
    Illegal PstI site found at 2023
    Illegal PstI site found at 2299
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 262
    Illegal EcoRI site found at 475
    Illegal BglII site found at 1976
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 262
    Illegal EcoRI site found at 475
    Illegal PstI site found at 991
    Illegal PstI site found at 1615
    Illegal PstI site found at 2023
    Illegal PstI site found at 2299
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 262
    Illegal EcoRI site found at 475
    Illegal PstI site found at 991
    Illegal PstI site found at 1615
    Illegal PstI site found at 2023
    Illegal PstI site found at 2299
    Illegal NgoMIV site found at 729
    Illegal NgoMIV site found at 951
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We codon optimized this gene for use in E. coli.


Source

http://www.ncbi.nlm.nih.gov/protein/Q9L4M7.1

References