Regulatory

Part:BBa_M36250:Design

Designed by: Evan Masutani and Erica Lieberman   Group: Stanford BIOE44 - S11   (2011-12-09)
Revision as of 09:04, 9 December 2011 by Emasutani (Talk | contribs) (→‎References)

PalkB inducible promoter activated by AlkS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing this part, efforts were made to extract only the promoter sequence while omitting the surrounding base-pairs as presented by van Beilen, et al., 2001. This may have proven to be a fatal flaw, as it is thought that the original surrounding sequences contribute to the functionality of the part as a whole.


Source

Van Beilen, J.B., S. Panke, S. Lucchini, A.G. Franchini, M. Rothlisberger, and B. Witholt. "Analysis of Pseudomonas Putida Alkane Degradation Gene Clusters and flanking Insertion Sequences : Evolution and Regulation of the Alk Genes." Microbiology 147 (2001): 1621-630.

References

Van Beilen, J.B., S. Panke, S. Lucchini, A.G. Franchini, M. Rothlisberger, and B. Witholt. "Analysis of Pseudomonas Putida Alkane Degradation Gene Clusters and flanking Insertion Sequences : Evolution and Regulation of the Alk Genes." Microbiology 147 (2001): 1621-630.

Makart, Stefan, Matthias Heinemann, and Sven Panke. "Characterization of the AlkS/PalkB-expression System as an Efficient Tool for the Production of Recombinant Proteins InEscherichia Coli Fed-batch Fermentations." Biotechnology and Bioengineering 96.2 (2007): 326-36.

Panke, Sven, Andreas Meyer, Caroline Huber, Bernard Witholt, and Marcel Wubbolts. "An Alkane-Responsive Expression System for the Production of Fine Chemicals." Applied Environmental Microbiology 65.6 (1999): 2324-332.