Composite
occupyDNAP

Part:BBa_M36549

Designed by: Rashmi Sharma, Bryce Bajar, Kendrick Wang   Group: Stanford BIOE44 - S11   (2011-12-08)
Revision as of 22:56, 8 December 2011 by Bbajar (Talk | contribs) (→‎Usage and Biology)

PoPS --> N4 gp8 (DNA replication inhibitor)

This composite part includes a medium-weak RBS, N4-derived gene (gp8), and a strong transcriptional terminator.

M36549.png

Usage and Biology

The coding region produces a small protein (gp8) which binds to the delta-subunit of DNA Polymerase III in the K12 strain of E. Coli to stop DNA replication.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//function/dna
//proteindomain/binding
Parameters
chassisE. coli
protein_outN4 gp8