Coding
N4 gp8

Part:BBa_M36548:Design

Designed by: Rashmi Sharma, Bryce Bajar, Kendrick Wang   Group: Stanford BIOE44 - S11   (2011-12-07)
Revision as of 20:59, 8 December 2011 by Bbajar (Talk | contribs) (→‎References)

DNA Polymerase Inhibitor (via N4 coliphage gp8)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We derived this sequence from coliphage N4 gp8. This produces a small protein which binds to the delta-subunit of DNA Polymerase III in E. Coli to stop DNA replication.

Source

Coliphage N4 gp8

References

Yano, S.T. and L.B. Rothman-Denes (2011), A phage-encoded inhibitor of Escherichia coli DNA replication targets the DNA polymerase clamp loader. Molecular Microbiology, 79: 1325-1338.