Composite

Part:BBa_M36295

Designed by: Dominique Dabija, Christopher Jackson, Debha Amatya   Group: Stanford BIOE44 - S11   (2011-12-06)
Revision as of 22:56, 6 December 2011 by Registry (Talk | contribs)

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Spider Silk GFP Actuator

This is the full sequence for the spider silk GFP actuator. It was the positive control to test if the FOS and JUN sequences worked. It includes the bicistonic sequence, FOS, a linker, GFP, another linker, JUN, a stop codon, and a transcription terminator. It is triggered by PoPS.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 109
    Illegal PstI site found at 151
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 109
    Illegal PstI site found at 151
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 109
    Illegal PstI site found at 151
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 109
    Illegal PstI site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 898


[edit]
Categories
Parameters
None