Coding
spilk 7.0.

Part:BBa_M36500:Design

Designed by: Dominique Dabija, Christopher Jackson, Debha Amatya   Group: Stanford BIOE44 - S11   (2011-12-03)
Revision as of 22:17, 3 December 2011 by Registry (Talk | contribs)

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Spider Silk Hexamer


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 254
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 254
    Illegal NotI site found at 46
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 254
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 254
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 407


Design Notes

Needed to minimize repeats and allow for ideal mRNA forming without too many loops while still maintaining codon optimization for E. coli.


Source

The monomer amino acid sequence was taken from Xia, X.-X., Z.-G. Qian, C. S. Ki, Y. H. Park, D. L. Kaplan, and S. Y. Lee. "Native-sized Recombinant Spider Silk Protein Produced in Metabolically Engineered Escherichia Coli Results in a Strong Fiber." Proceedings of the National Academy of Sciences 107.32 (2010): 14059-4063. Print.

References