Generator

Part:BBa_K511900

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)
Revision as of 15:56, 5 November 2011 by Chsuqed (Talk | contribs)

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Gal4-VP16 Transactivator Generator (Hef1a-Gal4-VP16) MammoBlock Device

This MammoBlock composite device produces the Gal4-VP16 transactivator at a low, constitutive level.

Graph showing the activation of UAS-Gal4_EYFP-4xFF4 by a constitutively expressed Gal4-VP16

Uas_eyfp-4xff4_hef1a_gv16.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal BglII site found at 615
    Illegal XhoI site found at 1014
    Illegal XhoI site found at 1726
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
    Illegal NgoMIV site found at 749
    Illegal AgeI site found at 127
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1645


[edit]
Categories
Parameters
n/aGal4-VP16 Transactivator Generator (Hef1a-Gal4-VP16) MammoBlock Device