Generator

Part:BBa_K511822

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)
Revision as of 15:55, 5 November 2011 by Chsuqed (Talk | contribs)

Inducible Yellow Fluorescent Protein Generator (UAS-Gal4-EYFP-FF4x4) MammoBlock Device

This MammoBlock composite device produces the yellow fluorescent protein EYFP-FF4x4 when induced with Gal4 transactivator variants and otherwise produces EYFP-FF4x4 at a low, basal (OFF) level. Note that the presence of the FF4 repeats on EYFP-FF4x4 means that this device can be repressed by the FF4 micro RNA.

Graph showing activation of the UAS_EYFP-FF4x4 by constitutively expressed Gal4-VP16. Uas_eyfp-4xff4_hef1a_gv16.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 126
    Illegal PstI site found at 447
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 126
    Illegal NheI site found at 154
    Illegal PstI site found at 447
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 126
    Illegal XhoI site found at 83
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 126
    Illegal PstI site found at 447
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 126
    Illegal PstI site found at 447
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aInducible Yellow Fluorescent Protein Generator (UAS-Gal4-EYFP-FF4x4) MammoBlock Device