Part:BBa_K515106

J23103 promoer - RBS B0034-RFP E1010 - Holin K112805 - endolysin K112806

A composite part comprising of a J23100 promotor, holin (BBa_K112805) and endolysin (BBa_K112806). This part has been to designed to lyse any cell which does not contain part BBa_K515104 in the genome.

Warning: This part is not RFC10 compatible

During Imperial College London's 2011 iGEM team's assembly of Gene Guard they encountered problems when cloning the holin-endolysin construct. They have found that there is an illegal XbaI site in the T4 Holin protein sequence. This was also found in the HQ's sequencing results but the RFC10 compatibility tag has not been removed from the front page of this part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 616
  Illegal AgeI site found at 728
  Illegal AgeI site found at 1730
  Illegal AgeI site found at 1800
  
• 1000
  COMPATIBLE WITH RFC[1000]