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Part:BBa_K553008:Experience

Designed by: Giulio Bernardinelli, Veronica Parisi, Francesca Cesaratto   Group: iGEM11_UNITS_Trieste   (2011-09-17)
Revision as of 10:21, 1 November 2011 by Giulio (Talk | contribs) (→‎Applications of BBa_K553008)

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Applications of BBa_K553008

UNITS_Trieste Synbiome project

bacteria Intra-strain communication: An E. Coli containing BBa_K553010 constitutively produces the OC12 AHL quorum sensing molecule. The ammount of this molecule is enough to induce the activation of part BBa_K553008 (production of OC8 AHL) in a co-cultivated bacteria.

The following assay demonstrates that the organic extract of a co-culture media contains a considerable ammount of OC8.

OC8 AHL standards used: 1uM; 100nM; 10nM; 1nM

Coculture assay.jpg

The diffusion from the center of the OC8 AHL generate, in fonction of its concentration, the activation of a biosensor (A. tumefacines) in Xgal LB agar dishes.

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