Status: 500 Content-type: text/html

Software error:

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Status: 500 Content-type: text/html

Software error:

syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 230, near ""<BR>$group_name, $year, $team_name, $team_digits, $range_name, $range_prefix")"
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

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aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator. This biobrick is constructed by deleting the anticodon recognition domain of AspRS from E.coli. This modified AspRS can charge Asp to tRNAAsp-TAG (BBa_K567013) and tRNAAsp-AGG (BBa_K567012).


To deprive AspRS of its anticodon specificity, we analyzed the structure of AspRS and expressed a truncated AspRS without anticodon recognition domain. Our design is shown below. This modified enzyme keeps its ability of aminoacylation while loses its activity of recognizing anticodon of tRNA.

Fig 1. The design for modification is based on the crystal structure of E.coli AspRS coupled with tRNA (PDB ID: 1C0A). This figure shows E.coli aspartyl-tRNA synthetase with and without anticodon recognition domain. After this domain is depleted, the enzyme cannot bind tRNAAsp anticodon. Yet the ability of aminoacylation is maintained.


Related Biobrick:

tRNAAsp-AGG (BBa_K567012)

tRNAAsp-TAG (BBa_K567013)

Sequence and Features Status: 500 Content-type: text/html

Software error:

syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 230, near ""<BR>$group_name, $year, $team_name, $team_digits, $range_name, $range_prefix")"
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

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Status: 500 Content-type: text/html

Software error:

syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 230, near ""<BR>$group_name, $year, $team_name, $team_digits, $range_name, $range_prefix")"
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
Compilation failed in require at /websites/parts.igem.org/cgi/extensions/part_box.cgi line 9.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/extensions/part_box.cgi line 9.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.