Part:BBa_K554015
flhDC HlyB HlyD TolC device
This part represents the final assembly of the hemolysin secretion system ([http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_3:_Secretion_system Device 3, Protein Secretion System]), very important to export the proteins produced inside bacteria and that must act in targets outside (such as IL-12 and IL-10). This device is composed by flhDC promoter linked to Hemolysin B, Hemolysin D, TolC and Terminator.
This is the Secretion device used by [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil UNICAMP-EMSE Brazil team] to export IL-12, since it is regulated by the promoter flhDC. The main purpose of not using constitutive production of this secretion system is to avoid energy waste, then bacteria would only produce these proteins under the specific signal (in this case, presence of Catecholamines or AI-3).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1394
Illegal BglII site found at 2057
Illegal BamHI site found at 4101 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 324
Illegal AgeI site found at 3561
Illegal AgeI site found at 3618 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3953
Usage and Biology
You can see a representation of this device acting in the schema below: HlyB, HlyD and TolC join together to produce the protein export system shown as a symbolic cilinder in orange.
Representation of device 3, the protein secretion system, in a Jedi bacteria that contains Device 1 (Adrenaline sensor/IL-12 producer). To export a protein, the bacteria must have the HlyD, HlyB and TolC proteins and the target protein must have a signal sequence (HlyA tail). In this case, the target protein to be secreted is IL-12.
A more realistic schema of ABC transport system is shown below:
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