Coding

Part:BBa_K530001

Designed by: Daniel Wolozny   Group: iGEM11_Johns_Hopkins   (2011-08-01)
Revision as of 21:09, 28 October 2011 by Danielw9 (Talk | contribs) (→‎Sequencing)

CRTE

Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Geranylgeranyl Diphosphate Synthase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 335
    Illegal XhoI site found at 34
    Illegal XhoI site found at 166
    Illegal XhoI site found at 985
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 202
  • 1000
    COMPATIBLE WITH RFC[1000]


Plasmid Map

CrtE Plasmid Map.png


Sequencing

CrtE Sequencing 2.png


This is the sequencing for colony 4.

[edit]
Categories
Parameters
None