Coding

Part:BBa_K525562

Designed by: Anna Drong   Group: iGEM11_Bielefeld-Germany   (2011-10-28)
Revision as of 14:53, 28 October 2011 by Registry (Talk | contribs)

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Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB with middle strong promoter,RBS

Fusion protein of ferredoxin-NADP+ oxidoreductase, BisdA and BisdB; RFC 25 (Freiburg BioBrick assembly standard) for characterization of intra- and extracellular BPA degradation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1411
    Illegal BamHI site found at 2149
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
    Illegal AgeI site found at 2402
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 746


[edit]
Categories
//chassis/prokaryote/ecoli
//function/degradation/bisphenol
//proteindomain/internal
Parameters
chassisE. coli
originSphingomonas bisphenolicum AO1