Composite

Part:BBa_K523025

Designed by: Chris French, Eugene Fletcher, Sylvia Ispasanie   Group: iGEM11_Edinburgh   (2011-10-28)
Revision as of 12:14, 28 October 2011 by Allancrossman (Talk | contribs)

Plac + RBS + cxnA (cex + cenA fusion)

Fusion of exoglucanse (cex) and endoglucanase (cenA) from Cellulomonas fimi under the control of the Lac promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1148
    Illegal NotI site found at 2846
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2759
    Illegal XhoI site found at 2257
    Illegal XhoI site found at 2506
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 781
    Illegal NgoMIV site found at 1154
    Illegal NgoMIV site found at 1656
    Illegal NgoMIV site found at 2037
    Illegal NgoMIV site found at 2962
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1201
    Illegal SapI.rc site found at 1284


[edit]
Categories
Parameters
None