Generator

Part:BBa_K649303:Experience

Designed by: Goshi Sugano   Group: iGEM11_Tokyo_Tech   (2011-09-19)
Revision as of 05:17, 28 October 2011 by Notothenia (Talk | contribs)

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Applications of BBa_K649303

isoprene synthesized by E. coli introduced ispS gene.
This work is done by Yuto Sugiuchi.
Each bacterial sample was grown in a 500 mL flask containing 100 mL LB media. Cultures were grown at 37℃ and then induced by 0.5 mM IPTG when OD600 reached 0.6. After 4 hours of induction, 50 mL of headspace gas was taken by absorbing material (mini-PAT including Tenax: Japan Analytical Industry Co., Ltd) and injected into GC-MS.

Each bacterial sample was grown in a 500 mL flask containing 100 mL LB media. Cultures were grown at 37℃ and then induced by 0.5 mM IPTG when OD600 reached 0.6. After 4 hours of induction, 50 mL of headspace gas was taken by absorbing material (mini-PAT including Tenax: Japan Analytical Industry Co., Ltd) and injected into GC-MS.

We calculated the amount of isoprene by [http://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/GC-Assay#AP calibration date we obtained].If X represents the area and Y represents the amount of isoprene [mg], the calibration curve is described by the equation,
" Y = 10-7.9 × X0.89 ".


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