Plasmid_Backbone

Part:BBa_J176128

Designed by: Brady Laughlin   Group: Haynes Lab   (2011-10-26)
Revision as of 21:51, 26 October 2011 by Registry (Talk | contribs)

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pcDNA3.1+ neo

pcDNA3.1+ neo/ ΔpCMV, based on pcDNA3.1+; no promoter; amp, neo

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
    Illegal PstI site found at 1627
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
    Illegal PstI site found at 1627
    Illegal NotI site found at 292
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal BglII site found at 12
    Illegal XhoI site found at 299
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
    Illegal PstI site found at 1627
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
    Illegal PstI site found at 1627
    Illegal NgoMIV site found at 737
    Illegal NgoMIV site found at 2078
    Illegal NgoMIV site found at 2361
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 3886
    Illegal SapI site found at 2806
    Illegal SapI.rc site found at 1927
    Illegal SapI.rc site found at 2137


[edit]
Categories
Parameters
n/apcDNA3.1+ neo