Part:BBa_K606040

Promoter Hyperspank B.subtilis & E.coli

Promoter hyper-spank is an IPTG inducible promoter that has been designed for high expression in B.subtilis. Gene expression under the control of the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG).

This promoter is flanked by two LacO sites. One downstream to make road block, and one upstream to prevent the binding of the sigma subunit. This part is similar but not exactly the same than the part K143055, with the noticable advantage that we sended the DNA to the registry.

Note: Since there is not a sufficient expression of the LacI gene in subtilis, we strongly recomand you to add a LacI expression cassette for subtilis, such as the one we designed https://parts.igem.org/Part:BBa_K606010 K606010] .

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]