Protein_Domain
mCh

Part:BBa_J176005

Designed by: Karmella Haynes   Group: Haynes Lab   (2011-09-29)
Revision as of 18:41, 25 October 2011 by Kahaynes (Talk | contribs)

mCh

Codon optimized mCherry red fluorescent protein


Usage and Biology

This "monomeric cherry" (mCherry) red fluorescent protein is codon optimized for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//classic/reporter
//function/reporter/fluorescence
Parameters
chassismammalian cells
emission610 nm
excitation587 nm