Generator

Part:BBa_K606027:Experience

Designed by: Kvin Yauy   Group: iGEM11_Paris_Bettencourt   (2011-07-27)
Revision as of 16:18, 24 October 2011 by Kyauy (Talk | contribs)


Characterization: Biobricked YFP:TetR

Parts construction:
Origins of YFP:TetR are from pFX234 of D. Lane (Toulouse 2 University).

Cloning plan of YFP:TetR construction



We characterize it with TetO Array from pDAG479 plasmid of F-X Barre and D. Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004).

Microscopy of double transformed pDAG470 / Biobricked YFP:TetR in E. Coli:

To characterize this part properly, we took pictures of different strains containing YFP:TetR alone and both TetO array (from pDAG479, D.Lane) and expression YFP:TetR system (BBa_K606027).

Biobricked YFP:TetR only
Biobricked expression YFP:tetR system in E. coli.
Biobricked expression YFP:tetR system in E. coli.
Biobricked YFP:TetR and TetO Array
Biobricked expression YFP:tetR system and TetO Array from D. Lane in E. coli.
Biobricked expression YFP:tetR system and TetO Array from D. Lane in E. coli.

The TetR-YFP construct which (emitter part) occasionally shows gross aggregated YFP.
After observing the cells carrying both the TetO and YFP:TetR constructs, we can obviously distinguish glowing dots in some cells. They reflect the behavior we expected.


More information on : TetO Array characterization (BBa_K606026) and iGEM Paris Bettencourt 2011 wiki [http://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion]