Generator
tRNAAmbsys

Part:BBa_K606065:Design

Designed by: Kvin Yauy   Group: iGEM11_Paris_Bettencourt   (2011-10-23)
Revision as of 17:40, 23 October 2011 by Kyauy (Talk | contribs) (→‎Design Notes)

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IPTG inducible tRNA Amber emitter with RFP monitor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 678
    Illegal AgeI site found at 790
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is the pure tRNA without the side sequences. This part was initially designed for B.subtillis but it also works in E.coli (look at our experience page BBa_K606034).

Source

Synthetize de novo by matching primers and cloned from the tRNA cluster of B. Subtilis genome of strain 168


References