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Part:BBa_K510022:Design

Designed by: David Caballero   Group: iGEM11_UPO-Sevilla   (2011-09-19)
Revision as of 15:32, 22 October 2011 by Dcabpra (Talk | contribs) (→‎Source)

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attTn7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The attTn7 site was flanked by prefix and suffix for cloning purposes.

Source

The attTn7 was obtained from the purified genome of E. coli MC4100 by PCR (using a high accuracy Pfu polymerase) with the follow primers: gaattcgcggccgcttctagagTGCAGCTGCTGGCTTACCATG and ctgcagcggccgctactagtaACATGGAGTTGGCAGGATGTTTG.

References

Ella Gringauz, Karina A. Orle, Candace S. Waddell, and Nancy L. Craig (1988). Recognition of Escherichia coli attTn7 by Transposon Tn7: Lack of Specific Sequence Requirements at the Point of Tn7 Insertion. Journal of Bacteriology, June 1988, p. 2832-2840.

Candace S. Waddellt and Nancy L. Craig (1989). Tn7 transposition: Recognition of the attTn7 target sequence. Proc. Natl. Acad. Sci. USA Vol. 86, pp. 3958-3%2, June 1989, Biochemistry. Joseph E. Peters and Nancy L. Craig (2001). Tn7: smarter than we thought. Nature reviews, November 2001, Vol. 2, 806.