Part:BBa_K633003
Extracellular Succrase Sacc
Sacc Extracellular Sucrase
This extracellular sucrase is an enzyme from Zymomonas mobilis. The expression product is different from SacB; showing sucrase but not levansucrase activity. In E. coli BL21, after induction of the T7 polymerase expression system with IPTG. Subcellular fractionation of the E. coli clone carrying plasmid pLSS2811 showed that more than 70% of the sucrase activity could be detected in the cytoplasmic fraction, suggesting that the enzyme was soluble and not secreted in E. coli. Its structure is monomeric, with an optimum temperature 20 to 40 ° C, pH of 2.5-7.5 and a size of 48 kDa. It also has a free N-terminus.
Kannan R, Mukundan G, Aït-Abdelkader N, Augier-Magro V, Baratti J, Gunasekaran P. Molecular cloning and characterization of the extracellular sucrase gene (sacC) of Zymomonas mobilis. Arch Microbiol. 1995 Mar;163(3):195-204.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 907
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 607
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