Part:BBa_K633000

CelD Mutated Cellulase

Clostridium thermocellum endoglucanase CelD was chosen as a representative member for detailed structural and functional studies of family E cellulases. Family E includes, beside C. thermocellum CelD, a number of cellulases of bacterial, fungal, and plant origin.

The corresponding gene, celD, has been overexpressed in Escherichia coli, and the enzyme is easily purified in large amounts from cytoplasmic inclusion bodies.

This specific cellulase sequence is the result of an investigation regarding activity, several mutations directed at the carboxilic residues (aspartate, glutamate), which are involved in the catalytic mechanism of celD, finally obtaining a celD with a 224% specific activity caused by the mutation Asp-523 ---> Ala on the peptide sequence.



Chauvaux, S., Beguin, P., & Aubert, J. (1992). Site-directed mutagenesis of essential carboxylic residues in clostridium thermocellum endoglucanase celd*. The Journal of Biological Chemistry, 267(5), 4472-4478. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1831
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 644
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 508
  Illegal AgeI site found at 151
  Illegal AgeI site found at 567
  Illegal AgeI site found at 1396
  
• 1000
  COMPATIBLE WITH RFC[1000]