![](https://parts.igem.org/images/partbypart/icon_device.png)
Part:BBa_K658004:Design
a bacteria population-control device with RBS0.3 driven by lacl+pL
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 718
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2345
Illegal BsaI.rc site found at 1874
Design Notes
This device is based on BBa_K658001. The RBS0.3 (BBa_B0032) in the killer protein producer regulate the expression of killer protein LacZalpha-ccdB. The higher strength a RBS has, the lower cell density may be got in the steady state.
Source
BBa_K658001
References
[1] You L, Cox RS, Weiss R, Arnold FH. Programmed population control by cell-cell communication and regulated killing[J]. Nature, 2004, 428(6985): 868-871.
[2] Eberhard A, Burlingame A. L, Eberhard C, Kenyon G. L, Nealson K. H,Oppenheimer N. J. Structural identification of autoinducer of Photobacterium fischeri luciferase[J]. Biochemistry ,1981,20 (9): 2444–2449.
[3] http://scholarworks.umass.edu/dissertations/AAI3397713/
[4] Gerdes K. Toxin-antitoxin modules may regulate synthesis of macromolecules during nutritional stress[J]. J. Bacteriol,2000,182 (3): 561–721.
[5]Philippe B,Martine C.Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes[J].Molecular Biology,1992,226(3):735-745.