Coding

Part:BBa_K559000

Designed by: Jacky, Fong Chuen, Loo   Group: iGEM11_Hong_Kong-CUHK   (2011-09-29)
Revision as of 21:11, 14 October 2011 by Jacky Loo (Talk | contribs)

Halorhodopsin

-Halorhodopsin, a light-driven ion pump originated from Halobacterium, employs light to transport chloride ions into cells uni-directionally against osmolality. -Halorhodopsin has the fascinated property to transform solar energy, the most abundant energy in the world, into intracellular chloride ion level.

This figure illustrates the molecular mechanism of Halorhodopsin gene on chloride ion absorption, in a controlled manner under light illumination.

HR mach.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 280
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 292
    Illegal AgeI site found at 18
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 228



Usage and Biology

We have use this biobrick to construct a functional halorhodopsin system unit
biology-NA-
device_type-NA-
function-NA-
protein-NA-


1. Essen, L.O. Halorhodopsin: light-driven ion pumping madesimple? Current opinion in structural biology 12, 516–522(2002)

[edit]
Categories
//cds/membrane/channel
//chassis/prokaryote/ecoli
//function/sensor/light
Parameters
biology
function
protein