Translational_Unit

Part:BBa_K622006:Experience

Designed by: Fumitaka Hashiya   Group: iGEM11_Kyoto   (2011-10-02)
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Applications of BBa_K622006

The function check of chitinase activity was performed by using DNS assay.

Since DNS assay is known as a method to detect the amount of reducing sugar, this assay was used to measure the quantity of N-acetylglucosamine, the main product of decomposition of chitin.

The chitinase activity was measured in the supernatant of culture. Figure 1 shows the amount of reducing sugar. From this result, no reducing sugar was observed and the chitinase activity couldn't observed.

Fig.1:

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