Device

Part:BBa_K629013:Design

Designed by: Zilong Wang   Group: iGEM11_SYSU-China   (2011-10-08)
Revision as of 09:26, 10 October 2011 by Skybird (Talk | contribs) (→‎Source)

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T7 promoter + lac operator + RBS + trkD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 61
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 346
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 61
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 61
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1421


Design Notes

the distance from RBS to ATG is 9bp.


Source

pET-32a, genome of E. Coli Bl21 Plys

References