Coding

Part:BBa_K632002

Designed by: Viki Ding   Group: iGEM11_Minnesota   (2011-09-28)
Revision as of 23:33, 9 October 2011 by NathanDavis (Talk | contribs) (→‎Usage and Biology)

INP-Silicatein

The silicatein gene has been successfully fused to an existent ice nucleation protein (BBa_K265009). Gel electrophoresis and DNA sequencing both suggest the fusion product is the correct size, frame, and orientation. Silicatein functional assay was performed to verify the activity of silicatein, and the results indicate the silicatein is enzymatically active.

Usage and Biology

The INP-Silicatein fusion protein is a chimeric protein with the outer membrane associated domain (N-terminal domain) of ice nucleation protein fused to silicatein which is capable of polymerizing silicic acid monomers. The 2011 University of Minnesota iGEM team generated the following data regarding this part:

Inp-silicatein standard curve.png Inp-silicatein results.png Inp-silicatein vials.png


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