Coding

Part:BBa_K632001

Designed by: Viki Ding   Group: iGEM11_Minnesota   (2011-09-28)
Revision as of 23:13, 9 October 2011 by Dingx055 (Talk | contribs)

INP-GFP

This part contains a fusion of an existent ice nucleation gene (BBa_K265009) and a GFP gene. GFP has been successfully fused to INP, verified by gel electrophoresis and DNA sequencing. The size of the product is correct and the sequence suggests it is in the correct orientation and frame. However, the cells with the INP-SIL fusion gene would not fluorescent. Spectrometer readings suggest limited fluorescence. We believe this construct is correct, but it needs to be tested in a different plasmid under the control of another promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 7
    Illegal PstI site found at 2113
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 2113
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 337
    Illegal XhoI site found at 1387
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 7
    Illegal PstI site found at 2113
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 7
    Illegal PstI site found at 2113
    Illegal NgoMIV site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None