Composite

Part:BBa_K631032

Designed by: Allister Smith   Group: iGEM11_Queens_Canada   (2011-09-15)
Revision as of 22:55, 9 October 2011 by AllisterSmith (Talk | contribs)

ODR7:ADBR2:UNC-54 (for use in C. elegans)

Composite part designed to modify chemotaxis of C. elegans.

Characterization

Part was characterized through the use of a chemotaxis assay. Agar plates were divided into four quadrants, 2 areas corresponding to 'target' and 2 'control' sections. In the target sections, differing concentrations of phenol were placed while control section used distilled water. Sodium azide was used as a paralytic in each quadrant to 'freeze' worms once they chemotaxed to that quadrant. <p> Worms were placed at the centre of the agar plate (inside circle) and were allowed to chemotax for one hour. After one hour, worms were counted in each quadrant. <p> Result<p> The results of the chemotaxis assay for the ODR-7:ADBR2:UNC-54 part are shown below.

Queens CanadaGraph.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1308
    Illegal BamHI site found at 2661
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1873


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Categories
Parameters
None