Composite

Part:BBa_K631032

Designed by: Allister Smith   Group: iGEM11_Queens_Canada   (2011-09-15)
Revision as of 22:55, 9 October 2011 by AllisterSmith (Talk | contribs)

ODR7:ADBR2:UNC-54 (for use in C. elegans)

Composite part designed to modify chemotaxis of C. elegans.

Characterization Part was characterized through the use of a chemotaxis assay. Agar plates were divided into four quadrants, 2 areas corresponding to 'target' and 2 'control' sections. In the target sections, differing concentrations of phenol were placed while control section used distilled water. Sodium azide was used as a paralytic in each quadrant to 'freeze' worms once they chemotaxed to that quadrant.

Worms were placed at the centre of the agar plate (inside circle) and were allowed to chemotax for one hour. After one hour, worms were counted in each quadrant.


Result The results of the chemotaxis assay for the ODR-7:ADBR2:UNC-54 part are shown below.

Queens CanadaGraph.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1308
    Illegal BamHI site found at 2661
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1873


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