Device

Part:BBa_K676002:Design

Designed by: Kheng Tee Ng   Group: iGEM11_UCL_London   (2011-09-09)
Revision as of 21:04, 9 October 2011 by Eintisar (Talk | contribs) (→‎Design Notes)

mNARK with YFP.LVA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

First Stage: Performed 3A assembly between mNark promoter (Part:BBa_K239006) and RBS (Part:BBa_B0034) and pSB1K3 plasmid backbone. Performed 3A assembly between YFP.LVA (Part:BBa_E0032) and terminator (Part:BBa_B0012) and pSB1T3 plasmid backbone.

Second Stage: Performed 3A assembly between mNark promoter/RBS composite part (Part:BBa_K239006 and YFP.LVA/Ter composite part (Part:BBa_B0034) and pSB1C3 plasmid backbone.

Stress Lights 2.0 Experiment.jpg

Source

mNarK promoter is designed to have better affinity to activated Fnr than the NarK promoter (BBa_K239005) and hence also stronger activity under anaerobic conditions. It is also shorter than the NarK promoter (BBa_K239005).

References