Generator

Part:BBa_K624058

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)
Revision as of 12:15, 9 October 2011 by Phonesents (Talk | contribs)

pYMB essentials + RBS(Pmsp3) + Inv

pYMB essentials + RBS(Pmsp3) + Inv

pYMB is a shuttle vector for manipulating and transforming gene into AMB-1. RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1. Inv(Invasin), is an outer membrane protein found on the surface of Y. enterocolitica that is responsible for its binding property. The ribosome binding site is from Pmsp3.(BBa_K624012)

The ribosome binding site is from Pmsp3.(BBa_K624012).

Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from Yersinia enterocolitica. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1938
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal NgoMIV site found at 2555
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None