Generator

Part:BBa_K624055

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)
Revision as of 11:19, 9 October 2011 by Peichinghuang (Talk | contribs)

pYMB essentials + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP

Pmsp1 + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP

The Pmsp1 is the second strongest promoter on AMB-1. RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1 tetR serves as a switch,which can turn on the downstream gene when Tetracycline is present ;and vice versa. GFP serves as a reporter to show if the construct work or not. All of the ribosome binding sites are RBS from Pmsp3.(BBa_K624012).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2561


[edit]
Categories
Parameters
None