Generator

Part:BBa_K624044

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)
Revision as of 11:17, 9 October 2011 by Peichinghuang (Talk | contribs)

pYMB essentials + RBS + LLO + RBS + Invasin + RBS + ECFP

ori + Pmsp1 + rep + RBS + LLO + RBS + Invasin + ECFP

The ori serve as the Origin of Replication,and the Pmsp1 is the second strongest promoter on AMB-1. LLO (Listeriolysin O) can make the bacteria be able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. which help protect the AMB-1 from extracellular immune system. ECFP serves as a reporter to show if the construct work or not.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2567
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3573
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal NgoMIV site found at 4190
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None