Device

Part:BBa_K629013:Design

Designed by: Zilong Wang   Group: iGEM11_SYSU-China   (2011-10-08)
Revision as of 04:01, 9 October 2011 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


T7 promoter + lac operator + RBS + trkD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 61
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 346
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 61
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 61
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1421


Design Notes

the distance from RBS to ATG is 9bp.


Source

pET-28a, genome of E. Coli Bl21 Plys

References