Composite

Part:BBa_K539741:Design

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-22)
Revision as of 10:41, 8 October 2011 by Bettyyang (Talk | contribs) (→‎Source)

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TetR repressible promoter (BBa_R0040)+ kivd (alpha-ketoisovalerate decarboxylase)+Ter (BBa_J61048)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 658
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1735
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Kivd is under tetR repressible promoter that is continuously turn ON or repressed by tetR.

Source

Kivd is cloned form Lactococcus lactis subsp, lactis KF147, by Zeng lab, NCTU-Formosa.

References