Composite
Part:BBa_K539741:Design
Designed by: Yang, Yi-Pei Group: iGEM11_NCTU_Formosa (2011-09-22)
TetR repressible promoter (BBa_R0040)+ kivd (alpha-ketoisovalerate decarboxylase)+Ter (BBa_J61048)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 658
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1735
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Kivd is under tetR repressible promoter that is continuously turn ON or repressed by tetR.
Source
Kivd is cloned form Lactococcus lactis subsp. lactis KF147