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Part:BBa_K539732:Design

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-24)
Revision as of 09:57, 8 October 2011 by Bettyyang (Talk | contribs) (→‎Source)

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kivd (alpha-ketoisovalerate decarboxylase) with native rbs(BBa_B0030) and terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 599
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1676
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is an available coding sequence that can be placed at the end of the circuit. We also made a similar biobrick that includes the different rbs .

Source

Kivd is cloned form Lactococcus lactis subsp, lactis KF147, by Zeng lab ,NCTU-Formosa.

References