Coding

Part:BBa_K539712:Design

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-20)
Revision as of 09:40, 8 October 2011 by Bettyyang (Talk | contribs) (→‎Source)

ilvD (dihydroxyacid dehydratase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1507
    Illegal BsaI site found at 1764


Design Notes

Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in coding sequence. There is one Pst1 in original sequence of ilvD. However, the RE site in ilvD has been modified by point mutation.
Ilvd.png

Source

The gene is cloned by Zeng lab in NCTU. It comes from Escherichia coli str. K-12 substr. MG1655

References