Generator

Part:BBa_K624051:Design

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)
Revision as of 03:52, 6 October 2011 by Phonesents (Talk | contribs) (→‎Source)

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pYMB essentials + RBS(trunc.) + LLO + RBS(trunc.) + Invasin + RBS(trunc.) + ECFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2558
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3555
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal NgoMIV site found at 4172
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

LLO: PCR amplification from the genomic DNA of Listeria monocytogenes

Inv: PCR amplification from the genomic DNA of Yersinia enterocolitica

References