Device

Part:BBa_K658010:Design

Designed by: Zhizhen Zhao   Group: iGEM11_XMU-China   (2011-10-01)
Revision as of 03:37, 6 October 2011 by Xmuwendy (Talk | contribs) (→‎Source)

a bacteria population-control device with lux pR-5 driven by lacl+pL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2347
    Illegal BsaI.rc site found at 1874


Design Notes

This device is based on BBa_K658001. The mutated promoter lux pR-5 in the killer protein producer regulate the expression of killer protein LacZalpha-ccdB. The strength of promoters lux pR and its 3 mutants lux pR-3, lux pR-5, lux pR-3/5 were tested before they were used as regulators in a circuit. The strength of the lux pR-5 is related to the expression of the killer protein LacZalpha-ccdB. The higher strength a lux pR has, the lower cell density might be got in the steady state.



Source

BBa_K658003

References