Deleted
Sample Discontinued
Experience: Works
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Device
iccdB1.0

Part:BBa_K658001:Design

Designed by: Yidan Hu   Group: iGEM11_XMU-China   (2011-09-20)
Revision as of 03:32, 6 October 2011 by Xmuwendy (Talk | contribs) (→‎Source)

a bacteria population-control device with RBS1.0 driven by lacl+pL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2344
    Illegal BsaI.rc site found at 1874


Design Notes

The bacteria population-control system was once a two-plasmid system. In order to enhance its stability and efficiency, we integrated target sequences into one plasmid.


Source

K176003

K658003

References

[1] Kampranis SC, Howells AJ, Maxwell A. The interaction of DNA gyrase with the bacterialtoxin CcdB: evidence for the existence of two gyrase-CcdB complexes[J]. Journal of Molecular Biology, 1999, 293(3): 733-744.

[2] Dwyer DJ, Kohanski MA, Hayete B, Collins JJ. Gyrase inhibitors induce an oxidative damage cellular death pathway in Escherichia coli[J]. Molecular Systems Biology, 2007, 3(1): 91. Galhardo RS, Do R, Yamada M, Friedberg EC, Hastings PJ, Nohmi T, Rosenberg SM. DinB upregulation is the sole role of the SOS response in stress-induced mutagenesis in Escherichia coli[J]. Genetics, 2009, 182(1): 55-68.