Device
IR-GFP

Part:BBa_K658016:Design

Designed by: Yidan Hu   Group: iGEM11_XMU-China   (2011-10-02)
Revision as of 03:17, 6 October 2011 by Xmuwendy (Talk | contribs) (→‎Design Notes)

LuxI->LuxR->lux pR->GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1874
    Illegal BsaI.rc site found at 2601


Design Notes

Similar to the definition of RBS strength, the strength of promoters lux pR and its three mutants could be tested using this device.

The device is constructed by combining two parts.

XMU China k658016F1.jpg

The two parts are proven to be right.


XMU China k658016F2.jpg

From the electrophoretogram,we can see that the part is successfully constructed.

Source

K658012


References