Part:BBa_K629003

cheZ, chemotaxis regulator, protein phosphatase for CheY

Background

Polar localization is dependent on CheA-short. CheZ is a cytosolic phosphatase which functions in the chemotaxis signal transduction complex. Plays an important role in bacterial chemotaxis signal transduction pathway by accelerating the dephosphorylation of phosphorylated CheY (CheY-P).

Expression in E. Coli

Before we started our functional test of CheZ, we have utilized western blot to determine whether there are enough soluble proteins in E. Coli. With 6×His tag on pET-32a, the result shows that CheZ has more expression with induction of IPTG.

Note: Because the promoter is Plac here, the expression has a background, which means control also expresses some cheZ without induction of IPTG.

Picture of Chemotaxis in cheZ knocked-out strain

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]